Monovalent ions modulate the flux through multiple folding pathways of an RNA pseudoknot

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Monovalent ions modulate the flux through multiple folding pathways of an RNA pseudoknot

1Department of Physics and 3Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607 2Department of Chemistry, University of Texas at Austin, Austin, TX 78712 #Present address: Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore MD 21218 ‡Present address: Division of Physics & Applied Physics, School of Physical and Mathematical Sciences, Na...

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How RNA sequences fold to specific tertiary structures is one of the key problems for understanding their dynamics and functions. Here, we study the folding process of an H-type RNA pseudoknot by performing a large-scale all-atom MD simulation and bias-exchange metadynamics. The folding free energy landscapes are obtained and several folding intermediates are identified. It is suggested that th...

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Predicting RNA pseudoknot folding thermodynamics

Based on the experimentally determined atomic coordinates for RNA helices and the self-avoiding walks of the P (phosphate) and C4 (carbon) atoms in the diamond lattice for the polynucleotide loop conformations, we derive a set of conformational entropy parameters for RNA pseudoknots. Based on the entropy parameters, we develop a folding thermodynamics model that enables us to compute the sequen...

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Folding 3-Noncrossing RNA Pseudoknot Structures

In this article, we present the novel ab initio folding algorithm cross, which generates minimum free energy (mfe), 3-noncrossing, canonical RNA structures. Here an RNA structure is 3-noncrossing if it does not contain three or more mutually crossing arcs and canonical, if each of its stacks has size greater or equal than two. Our notion of mfe-structure is based on a specific concept of pseudo...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2018

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.1717582115